疫霉属（Phyt）- TAS ELISA检测试剂盒

Genus: Phytophthora

Main host plants and diseases: Wide range of host plants including many

important crops.

Transmission: Air-born sporangia, infected plant tissues, seed, tuber, soil

and agricultural activities.

Sensitivity: Sensitivity of the ELISA is moderately high. The fungus can be

consistently detected in infected plant tissues diluted at 1:270 - 1:810.

Reactivity: This ELISA detects at least the following 16 Phytophthora species: P. cactorum, P. cambivora, P. capsici, P. cinnamomi, P. citricola,

P. citrophthora, P. cryptogea, P. drechsleri, P. erythroseptica, P. fragariae,

P. infestans, P. megasperma, P. palmivora, P. parasitica, and P. sojae. It

reacted with all 39 isolates of Phytophthora infestans tested. Reaction

of this test is moderately strong. Optical Density at 405 _nm is in a range

of 0.480 - 1.800 depending on the fungal titers in the samples tested.

Specificity: This test system reacts negatively with the following species of

fungi: Pythium aphanidermatum, P. catenulatum, P. heterothalicum,

P. irregulare, P. oligandrum, P. spinosum, P. splendens, P. torulosum,

P. ultimum, P. ultimum var. ultimum. The test did not react with

isolates of Aspergillu ssp., Fusarium sp., Monilinia sp., Penicillium sp.,

Rhizoctonia sp., and Sclerotinia sp. There are moderate cross-reaction

occurring with pure culture extracts of Pythium aristosporum,

P. arrhenomanes, P. graminicola, P. paroecandrum, and P. violae, and weak

cross-reaction occurring with pure culture extracts of Pythium multisporum,

P. myriotylum, P. sylvaticum, P. tardicrescens, P. vanterpoolii. There is no

cross reaction with healthy plant tissues. Background is low on all of the

negative control wells.