硝基呋喃（SEM） Nitrofuran (SEM)- 残留检测

Cat#:SS33

Catalog	Components	Quantity
SS33-K1	Nitrofuran (SEM) ELISA Test Kit	96
SS33-K2	Nitrofuran (SEM) ELISA Test Kit	192
SS33-K3	Nitrofuran (SEM) ELISA Test Kit	288

Sensitivity: 0.1 ppb
Detection limit: Tissue, honey, egg 0.2 ppb; Fish, shrimp (some interference in shrimp and fish) 0.3 ppb
Recovery Rate: Shrimp and fish 85±10%; Honey, meat, liver, egg 75±15%
Cross-reation rate: SEM 100%; AHD ﹤0.1%; AMOZ ﹤0.1%; AOZ ﹤0.1%

Brief: Furacili is synthetic broad-spectrum antibiotic and can treat animal desease widely , which is frequently

employed in animal and aquaculture production. But Furacilin and its metabolite residues in animal food are passed to human through the food chain , taking them regularly cause various diseases, which can lead to cancer,

teratogenesis and other side effects. Furacilin was banned from use in food industry in Australia, Canada, Japan,

Singapore, and analsysis of Nitrofuran was strictly implemented in Aquatic Products. Japanese positive list regulated that Nitrofuran medicine mustn’t be detected out. The use of Nitrofurantoin was are classified as forbidden drugs

in Veterinary medicine And Other Compound Detailed Banned in Food Animal Lists which was pulished by Ministry of Chinese Agriculture in March 2002.
SEM-residues are determined most commonly by HPLC, LC-MS before. Enzyme immunoassays, compared with

chromatographic methods, show quite advantages regarding sensitivty , simple process , technical equipment and

time requirement.

Principle: The Semicarbazide (SEM) ELISA test Kit is based on the competitive enzyme immunoassay for the

detection of Semicarbazide (SEM) in the tissue(chicken, fish, shrimp), milk, honey, whole egg and so on. The

coupling antigen is pre-coated on the micro-well stripes. The SEM in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against SEM. After the addition of the enzyme

conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample is

inversely proportional to the SEM in the sample. This value is compared to the standard curve and the content of the corresponding SEM is subsequently obtained.