豆薯层锈菌(Ppac) - TAS ELISA检测试剂盒

Sensitivity: This ELISA is very sensitive, and can detect the ASR fungus in

pre‐symptomatic plant leaves. The ASR pathogen can be detected at

dilution of 1:21870 for the plant leaf samples prepared at 14 days after

infection, and at dilution of 1:270 for the plant leaf samples prepared at

5 days after infection.

Reactivity: This ELISA reacts specifically with Phakopsora pachyrhizi fungus. The assay detected the isolates of P. pachyrhizi in infected host plants such

as soybean and lima bean.

Reaction of this test is very strong. Optical Density at 405 _nm is in a range of 1.000 - >3.000 depending on the fungal titers in the samples tested.     

Specificity: This test system is species‐specific for P. pachyrhizi. This ELISA

assay only reacts with the isolates of P. pachyrhizi species, and did not

react with P. meibomiae. The antibodies used in this ELISA have high

specificity to r369, and did not react with r107 protein.

This assay does not react with the other related rust fungi or any of the

soybean pathogens tested, including Uromyces appendisulatus, Puccinia

punctiformis, Puccinia graminis, Puccinia graminis tritici, Rose rust,

Fusarium graminearum, Downy mildew, Pseudomonas syringae ,

Pseudomonas syringae pv. phaseolicola, Xanthomonas campestris pv.

glycine, Ustilago tritici, Erysiphae polygono DC, Septoria lycopersici and

Cercospora sojina.

There is no cross reaction with healthy plant tissues. Background is very low on all of the negative control wells.