柄锈菌属 (Phak) - TAS ELISA 检测试剂盒

Sensitivity: This ELISA is very sensitive, and can detect ASR/SR fungi in

pre‐symptomatic plant leaves. The ASR/SR pathogen can be detected at

dilution of 1:21870 for the plant leaf samples prepared at 14 days after

infection, and at dilution of 1:270 for the plant leaf samples prepared

at 5 days after infection.

Reactivity: This ELISA reacts with P. pachyrhizi and P. meibomiae fungal

species. The assay detected the isolates of P. pachyrhizi and P. meibomiae 

ininfected host plants such as soybean and lima bean.

Reaction of this test is very strong. Optical Density at 405_nm  is in a range of 2.000 - >3.000 depending on the fungal titers in the samples tested.

Specificity: This test system is genius‐specific for Phakopsora, and reacts

with both species of P. pachyrhizi (ASR) and P.meibomiae (SR). The

antibodies used in this ELISA have high specificity to both r369 and r107

proteins.

This assay does not react with the other related rust fungi or any of the

soybeanpathogens tested, including  Uromyces appendisulatus, Puccinia

punctiformis, Puccinia graminis, Puccinia graminis tritici, Rose rust, 

Fusarium graminearum, Downy mildew, Pseudomonas syringae ,

Pseudomonas syringae pv. phaseolicola, Xanthomonas campestris pv.

glycine, Ustilago tritici, Erysiphae polygono DC, Septoria lycopersici and 

Cercospora sojina.

There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.